What is Laminar Air Flow Cabinet, It’s Principle and Uses?

Laminar Air Flow Cabinet is preferably known as an enclosed workstation whose main purpose lies in creating an environment which is work free. The laminar air flow cabinet makes use of filters which helps in capturing all the particles which make their entrance within the cabinet.

? These cabinets are highly useful in a work free environment. Laminar Air Flow Cabinets are specifically designed for the distribution of plate pouring and specific media
? These cabinets are similar to bio-safety cabinets. The only difference
is that in the laminar air flow cabinets, the air is drawn to the users
face
? Another major difference between the laminar air flow cabinet and bio-safety cabinet is that in the bio-safety cabinet both the users as well as the sample are protected whereas within the laminar flow cabinet, only the sample is secured

WORKING OF A LAMINAR AIR CABINET HOOD

?? The working of an air flow cabinet is highly based on the principle of laminar air flow
?? The device operates with an inward flow of air within one or more
HEPA filters to produce a work free environment
?? In the next step, the air is then further taken along a filtration system
followed by the exhaustion pass the work surface which is a part of the laminar flow of the air
?? The air is passed along a filter pad which enables an efficient flow of air into the cabinet
?? The blower or the fan of the laminar air flow cabinet directs the air
towards the HEPA filters
?? The HEPA filters are useful in identifying and capturing the fungi,
bacteria as well as other materials to ensure a clear work free environment
?? The pollutant air of the cabinet is passed through the perforation
which is placed at the bottom rear end of the device
?? There is a constant positive air pressure which prevents the entrance
of the contaminated external air

BENEFITS OF USING A LAMINAR AIR FLOW HOOD

? This cabinet is used in laboratories for carrying out sensitive processes such as the plant tissue process
? Laminar Air Flow Cabinet is also used to conduct the processes such as identifying the culture of organisms, media plate preparation
? The cabinet is compatible in performing the operations of sensitive electronic devices
? Useful in microbiological and industrial sectors
? The drug preparation techniques within the pharmaceutical
industries can also be performed inside the cabinet

UNITS OF A LAMINAR AIR HOOD:

  1. The Cabinet:
    ? Made of stainless steel with no gaps, preventing any contaminated particle
    ? Provides a medium of neutralizing within the inner environment of the cabinet
  2. WORKING STATION:
    ? A compatible working station is present within the cabinet to undertake all the processes within the cabinet
    ? Made of stainless steel which prevents rusting
    ? The burner, loops, plates are present within the cabinet for all the processes to take place
  3. PRE-FILTER OR FILTER PAD:

? Present on top of the cabinet for the air to easily pass
? Attracts the dust particles for a clear work environment

  1. BLOWER:

? Consumes the air and moves it across the entire cabinet
? Allows the direction of the air along the HEPA filter

  1. UV LAMP:

? Helpful in sterilizing the interiors of the cabinet before and
after the operation
? Prevents the harmful rays of light on the body of the user.
Should be turned on 15 minute before the operation

  1. THE HEPA FILTER:

? The High Efficiency Particulate Air Filter makes the
operations more smooth and is present within the cabinet
? Useful in trapping the unwanted bacteria, fungi through the pre
filtered air
? Reduces the chance of contamination

POINTS TO CONSIDER:

? Sterilization with the UV lights both before and after the operation
? It is integral to note that both the UV light and the airflow cannot be used together at once
? Avoid the process of any operation while the UV light is turned on
? Lab dress and gloves are essential for the operator
? Sterilize the glass shield, working bench along with other components both before as well as after the completion of the work

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